부메뉴
자료실
Enantiomeric Separation of Cinacalcet hydrochloride
신고
인쇄
스크랩
Chromatographia 2009, 70, July (No. 1/2)
DOI: 10.1365/s10337-009-1129-5
Cinacalcet hydrochlride은 CHIRALPAK IA로 키랄 분석이 가능합니다. 상세 내용은 첨부의 문헌을 통해 확인할 수 있습니다.
A Validated Chiral LC Methodfor the Enantiomeric Separation of Cinacalcet Hydrochloride
A rapid isocratic chiral LC method has been developed for the separation of (S)-cinacalcet fom (R)-cinacalcet. Good resolution with RS > 3 was obtained using a Chiralpak-IA column (250 9 4.6 mm, particle size 5 lm) and n-hexane, ethanol and trifluoroacetic acid as the mobile phase (95:5:0.1, v/v) at ambient temperature. Flow rate was kept at 1.0 mL min–1 and elution was monitored by UV detection at 223 nm. This method was further used to
determine the presence of (S)-cinacalcet in enantiopure pharmaceutical formulations containing (R)-cinacalcet. This method allowed for the detection and quantitation of (S)-cinacalcet of levels at 0.04 and 0.16 lg mL–1, respectively. The method was validated following ICH guidelines.
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